What is the purpose of sodium Polyanethol sulfonate?

Sodium polyanethole sulfonate (SPS; trade name, Liquoid) is a constituent in culture media used to grow bacteria from blood samples from patients suspected of bacteremia. SPS prevents the killing of bacteria by innate cellular and humoral factors.

What is anticoagulant SPS?

Sodium polyanetholesulfonate (SPS), an anticoagulant which inhibits the antimicrobial systems of blood, is used widely in blood culture media. The addition of SPS to experimental blood cultures inoculated with small numbers of a variety of organisms caused a striking increase in recovery of these organisms.

Is sodium Polyanethol sulfonate an anticoagulant?

Sodium polyanethole sulfonate (SPS) is the most common anticoagulant used in commercial blood culture bottles. SPS has been shown to function as an anticoagulant (4) and as an inhibitor of humoral and cellular elements that might interfere with bacterial growth.

What is an anaerobic blood culture?

Blood cultures are drawn into special bottles that contain a special medium that will support the growth and allow the detection of micro- organisms that prefer oxygen (aerobes) or that thrive in a reduced-oxygen environment (anaerobes).

What do positive blood cultures mean?

A blood culture is a test that checks for foreign invaders like bacteria, yeast, and other microorganisms in your blood. Having these pathogens in your bloodstream can be a sign of a blood infection, a condition known as bacteremia. A positive blood culture means that you have bacteria in your blood.

What is BacT alert?

Abstract. BacT/Alert (Organon Teknika Corp., Durham, N.C.) is an automated microbial detection system based on the colorimetric detection of CO2 produced by growing microorganisms.

Is sodium citrate an anticoagulant?

Background: Sodium citrate has been used as an anticoagulant to stabilize blood and blood products for over 100 years, presumably by sequestering Ca(++) ions in vitro.

What is the liquid in blood culture bottles?

Blood culture bottles contain a growth medium, which encourages microorganisms to multiply, and an anticoagulant that prevents blood from clotting. Sodium polyanethol sulfonate (SPS) is the most commonly used anticoagulant because it does not interfere with the growth of most organisms.

Which blood culture bottle is more important?

aerobic bottle
The aerobic bottle is the more critical one to inoculate short samples into. The small lines on the edge of the label indicate approximately 5 cc and there is a fill line denoted on the label. It is important not to underfill or overfill the bottles as this can adversely affect the results.