Can a 1 mL loop cause dead volume?
The loop can be a significant source of HPLC system dead volume. There is no reason to have a 1 mL loop when the injection volume is 10 Ls. A large loop volume will delay the peak and possibly adversely affect your chromatography.
Why do I need a large loop volume?
A large loop volume will delay the peak and possibly adversely affect your chromatography. For a proper under fill, 10 µLs of a precious sample can be safely made onto a 25 µL loop. In this latter example the tailored loop volume helps to cut down on surface area, where a dilute sample may adsorb,…
What are the dimensions of an injection loop?
Loop dimensions are important. The most accurate injections are made with a technique known as overfilling. When using the overfill technique a sample injection will be made that is 3 to 5 times larger than the volume of the injection loop.
Which is the formula for the volume of a cylinder?
Cylinder’s volume is given by the formula, πr 2 h, where r is the radius of the circular base and h is the height of the cylinder. The material could be a liquid quantity or any substance which can be filled in the cylinder uniformly.