What is the use of potassium ferricyanide?

In histology, potassium ferricyanide is used to detect ferrous iron in biological tissue. Potassium ferricyanide reacts with ferrous iron in acidic solution to produce the insoluble blue pigment, commonly referred to as Turnbull’s blue or Prussian blue.

What does Drabkin’s reagent form when mixed with hemoglobin?

Drabkin’s Solution reacts with all forms of hemoglobin except sulfhemoglobin, a pigment that normally occurs in only minute concentrations in blood. This procedure is based on the oxidation of hemoglobin and its derivatives (except sulfhemoglobin) to methemoglobin in the presence of alkaline potassium ferricyanide.

How is hemoglobin measured in hematology analyzer?

The hemoglobin is converted to a form called cyanmethemoglobin after addition of cyanide and the concentration is read by a spectrophotometer with the wavelength set at the peak absorbance of cyanmethemoglobin (around 540 nm). The concentration of hemoglobin is then measured from the absorption of the solution.

What is the basic principle of Cyanmethemoglobin determination of hemoglobin?

The cyanmethemoglobin method works on the principle of conversion of hemoglobin to cyanmethemoglobin by the addition of potassium cyanide and ferricyanide whose absorbance is measured at 540 nm in a photoelectric calorimeter against a standard solution [16].

How do you neutralize potassium ferricyanide?

An alkaline solution of potassium ferricyanide, however, is reduced to potassium ferrocyanide by hydrogen peroxide, with evolution of oxygen: 2K3FeC6N6 + 2KOH + H2O2 = 2K4FeC6N6 + 2H2O + O2.”

Is potassium ferricyanide hazardous?

Potassium ferrocyanide is apparently benign and does not decompose to cyanide in the body. Inhalation: May cause irritation to the respiratory tract. Symptoms may include coughing and shortness of breath. Ingestion: Large doses may cause gastrointestinal upset with nausea, vomiting, diarrhea, and possible cramping.

How do you prepare a hemoglobin solution?

Drabkin’s solution reagents needed are:

1. Potassium ferricyanide = 200 mg.
2. Potassium cyanide = 50 mg.
3. Potassium dihydrogen phosphate = 140 mg.
4. Distal water = Make up to 1000 ml (1 L)

What enzyme converts methemoglobin to hemoglobin?

The NADH-dependent enzyme methemoglobin reductase (a type of diaphorase) is responsible for converting methemoglobin back to hemoglobin.

How is total hemoglobin calculated?

For a hemoglobin test, a member of your health care team takes a sample of blood by pricking your fingertip or inserting a needle into a vein in your arm. For infants, the sample may be obtained by pricking the heel. The blood sample is sent to a lab for analysis.

What is normal range of hemoglobin?

Normal results for adults vary, but in general are: Male: 13.8 to 17.2 grams per deciliter (g/dL) or 138 to 172 grams per liter (g/L) Female: 12.1 to 15.1 g/dL or 121 to 151 g/L.

What is the clinical significance of determining hemoglobin concentration?

Hemoglobin concentration (Hb) is used clinically to determine the presence of anemia, which is functionally defined as insufficient red blood cell (RBC) mass to adequately deliver oxygen to peripheral tissues.

Which hemoglobin is detected by Sahli’s method?

Sahli´s Method Sahli’s hemoglobinometer is a manual device that contains a hemoglobin tube, pipette, and stirrer, as well as a comparator. Hydrochloric acid converts hemoglobin to acid hematin, which is then diluted until the color of the solution matches that of the comparator block.

What kind of ferricyanide is used to measure hemoglobin?

Hemoglobin conversion via potassium ferricyanide to the colored, stable azide methemoglobin form that has an almost identical absorbance spectrum as HiCN, which represents the key characteristic of Vanzetti’s azide methemoglobin method. 2

How is methemoglobin oxidized by potassium ferricyanide?

In the cyanmethemoglobin method, blood is diluted in an alkaline Drabkin solution of potassium ferricyanide, potassium cyanide, sodium bicarbonate, and a surfactant. The hemoglobin is oxidized to methemoglobin (Fe 3+) by the potassium ferricyanide, K 3 Fe (CN) 6.

What is the absorbance of potassium ferricyanide?

Potassium ferricyanide converts hemoglobin to methemoglobin, which is converted to cyanmethemoglobin by the action of potassium cyanide. The absorbance was measured colorimetrically at 540 nm.

What happens when you add potassium cyanide to hemoglobin?

Following RBC lysis, potassium ferricyanide oxidizes the ferrous ion of hemoglobin to the ferric ion of methemoglobin. Methemoglobin is then converted to cyanomethemoglobin following the addition of potassium cyanide. Absorbance at 540 nm is measured, and the intensity of this signal corresponds to hemoglobin concentration [5].